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Inductive effect of recombinant human interleukin‐1 α and β on differentiation of macrophage‐like tumor cell line P388D1
Author(s) -
Hanazawa Shigemasa,
Hanaizumi Chika,
Amano Shigeru,
Hirose Kimiharu,
Ohmori Yoshihiro,
Kumegawa Masayoshi,
Yamaura Kohichi,
Kitano Shigeo
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041360322
Subject(s) - cell culture , microbiology and biotechnology , egta , cell growth , monocyte , cytokine , macrophage , chemistry , biology , in vitro , biochemistry , calcium , immunology , genetics , organic chemistry
We used the mouse monocyte/macrophage‐like tumor cell line P388D1 to test whether or not interleukin‐1 (IL‐1) stimulates differentiation of monocyte/macrophage progenitors. Incubation of these cells with recombinant human interleukin‐1 (rhIL‐1) α and β resulted in their increased adherence, stimulation of nonspecific esterase activity, and increased Fc rosette formation. rhIL‐1s inhibited cell growth and stimulated Fc rosette formation in a dose‐dependent fashion. The cell growth inhibition due to rhIL‐1s depended on the concentration of serum in culture medium. Synergism between rhIL‐1 and calcium ionophore A23187 was found for the cell growth inhibition and Fc rosette formation. The presence of ethylene glycol bis‐ (β‐aminoethyl ether) N,N,N,N‐tetraacetic acid(EGTA) in the medium abolished the stimulatory effect of rhIL‐1 on Fc rosette formation of the cell line. These results demonstrate that rhIL‐1s are a potent inducer of the differentiation of the macrophage‐like tumor cell line P388D1.