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Differential effect of D‐penicillamine on the cell kinetic parameters of various normal and transformed cellular types
Author(s) -
Friteau L.,
Jaffray P.,
Ronot X.,
Adolphe M.
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041360317
Subject(s) - hela , cell cycle , penicillamine , flow cytometry , cell growth , cell , mitosis , microbiology and biotechnology , cytokinesis , dna synthesis , chemistry , cell culture , cell division , mitotic index , biology , dna , biochemistry , genetics , organic chemistry
The cell‐growth‐inhibitory and phase‐specific effects of D‐penicillamine on cell‐cycle progression were investigated using cell‐proliferation patterns, quantitative cell‐cycle analysis by flow cytometry, and determination of the mitotic index and binucleate cell fraction of normal (rabbit articular chondrocytes, L 809, rabbit fibroblasts) and transformed (HeLa, L 929) cells. D‐penicillamine treatment resulted in an inhibition of growth within a dose range of 5 × 10 −4 M to 7.5 × 10 −3 M. Examination of DNA by flow cytometric analysis revealed that rabbit articular chondrocytes were preferentially arrested in the G0/1 phase of the cell cycle, whereas the other cell lines were blocked in the G2 + M phase; the increase in the proportion of cells with G2 + M DNA content was partially due to an enhancement of binucleate cells, resulting in a cytokinesis perturbation for HeLa and L 929 cells. These results showed that D‐penicillamine affects cell proliferation through different events according to cell type.