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Superoxide as a signal for increase in intracellular pH
Author(s) -
Shibanuma Motoko,
Kuroki Toshio,
Nose Kiyoshi
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041360224
Subject(s) - superoxide , butylated hydroxyanisole , superoxide dismutase , xanthine oxidase , chemistry , intracellular , biochemistry , xanthine , u937 cell , amiloride , antioxidant , microbiology and biotechnology , biology , enzyme , sodium , apoptosis , organic chemistry
We examined the role of superoxide in the increase in intracellular pH (pHi) of human histiocytic leukemia U937 cells treated with 4β‐phorbol‐12,13‐didecanoate (4β‐PDD) or serum. 4β‐PDD or serum induced a rapid increase in pHi, and antioxidants such as superoxide dismutase (SOD), vitamin E, and butylated hydroxyanisole (BHA) were found to inhibit the amiloride‐sensitive increase in pH i induced by 4β‐PDD. SOD inhibited the increase in pHi caused by serum, and essentially the same was found in concanavalin A‐stimulated mouse thymocytes. Also, a superoxide‐generating system, xanthine‐xanthine oxidase (X‐XOD), increased pH i of U937 cells as much as 4β‐PDD or serum. From these findings, it appears that superoxide is the basis for the modulation of pH i .