Premium
Regulation of neuroblast proliferation by hormones and growth factors in chemically defined medium
Author(s) -
Wu Doris K.,
Maciag Thomas,
de Vellis J.
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041360222
Subject(s) - thymidine , neuroblast , hormone , biology , growth factor , cell growth , endocrinology , medicine , triiodothyronine , cell culture , chemically defined medium , microbiology and biotechnology , biochemistry , in vitro , genetics , neurogenesis , receptor
Pure neuronal cultures prepared from 6‐day‐old embryonic chick brains incorporated [ 3 H]‐thymidine in serum‐free medium up to the 4th day in culture. The addition of insulin any time within this culture period caused an increase in thymidine incorporation. This increase in [ 3 H]‐thymidine was correlated with an increase in cell number and percentage of labeling index. Triiodothyronine and endothelial cell growth factor were also active in stimulating [ 3 H]‐thymidine incorporation into chick neuroblasts. The effect of these trophic agents is unique since a variety of known mitogens tested were negative.