z-logo
Premium
Bone marrow stromal proteoglycan heterogeneity: Phenotypic variability between cell lines and the effects of glucocorticoid
Author(s) -
Bentley Stuart A.,
Kirby Suzanne L.,
Anklesaria Pervin,
Greenberger Joel S.
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041360124
Subject(s) - proteoglycan , stromal cell , chondroitin sulfate proteoglycan , cell culture , haematopoiesis , chondroitin sulfate , chemistry , bone marrow , microbiology and biotechnology , biology , biochemistry , glycosaminoglycan , stem cell , immunology , extracellular matrix , cancer research , genetics
Hematopoiesis in vivo is dependent upon the interaction of hematopoietic stem cells with a complex microenvironment, of which stromal proteoglycans are an important functional component. Certain bone marrow stromal cell lines provide a microenvironment that supports hematopoiesis in vitro, a function that is dependent upon glucocorticoid supplementation. Proteoglycan synthesis in the hematopoietic‐supportive D2XRll, Bl6 and 14F1 bone marrow stromal cell lines was studied by 35 S‐sulfate precursor labelling and ion‐exchange separation, followed by isopyknic CsCl density centrifugation and gel filtration HPLC. The effects of glucocorticoid were also investigated. A similar pattern of proteoglycan heterogeneity was observed in all three cell lines, although there was considerable quantitative variation. All cultures synthesized three species of chondroitin/dermatan sulfate (CS/DS) proteoglycans: DS1, excluded from a Bio‐Sil TSK‐400 HPLC column, and DS2, eluting at Kd = 0.31, were present mainly in the culture media. The smallest (DS3) eluted at Kd = 0.63 and was present mainly in the cell layers. CS/DS species were the major proteoglycans in all cultures. Hydrocortisone‐free cultures also synthesized heparan sulfate (HS) proteoglycans, including a cell‐associated form (HS1), partially excluded from the TSK‐400 column, and a secretory form (HS2), eluting at Kd = 0.15. D2XRll cells also secreted an apparently‐unique, high‐density proteoglycan, Kd = 0.65, into the culture medium. Hydrocortisone at 10 −6 M virtually abolished HS proteoglycan synthesis in all three cell lines, and altered the pattern of CS/DS proteoglycans in the culture media, increasing the quantity of DS1 and DS3, and reducing the quantity of DS2.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here