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Norepinephrine modulates the growth‐inhibitory effect of transforming growth factor‐beta in primary rat hepatocyte cultures
Author(s) -
Houck Keith A.,
Cruise Jennifer L.,
Michalopoulos George
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041350327
Subject(s) - prazosin , hepatocyte , endocrinology , antagonist , medicine , adrenergic receptor , dna synthesis , transforming growth factor beta , norepinephrine , beta (programming language) , receptor , inhibitory postsynaptic potential , transforming growth factor , chemistry , biology , in vitro , biochemistry , dopamine , computer science , programming language
TGF‐beta is a potent inhibitor of EGF‐induced DNA synthesis in primary rat hepatocyte cultures. Norepinephrine (NE) was shown to modulate this inhibition of DNA synthesis. It produced a five‐fold increase, from 2.8 pM to 14.4 pM, in the ID 50 for TGF beta. The effect was dose‐dependent and was significant at concentrations of 10 −6 M NE and greater. The modulation by NE was mediated by the alpha 1 ‐adrenergic receptor as shown by the ability of the alpha 1 antagonist prazosin to block the activity. This effect might be important during liver regeneration in allowing escape of hepatocytes from negative growth control exerted by TGF‐beta.