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Retinoic acid inhibits Ca 2+ currents and cell proliferation in a B‐lymphocyte cell line
Author(s) -
Bosma Martha,
Sidell Neil
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041350220
Subject(s) - patch clamp , retinoic acid , cell growth , cell culture , biophysics , chemistry , microbiology and biotechnology , biology , biochemistry , receptor , genetics
Using the whole‐cell variation of the patch‐clamp technique, we have demonstrated that retinoic acid (RA) blocks Ca channels and inhibits cell proliferation in a mouse hybridoma cell line (MHY206) derived from a fusion of murine myeloma and splenic B cells. In 25 mM external Ca, and with an Na internal solution containing aspartate, cAMP, and Mg‐ATP, inward currents were activated in these cells from holding potentials more negative than −70 mV, peaked at voltage steps up to −20 mV, and were voltage‐inactivated within the 125‐msec duration of the pulse. With more positive pulses, outward current carried by Na ions permeating through the Ca channels were seen. Application of RA blocked both inward and outward current through the Ca channels in a dose‐dependent manner, with 50% block at a concentration of around 5 × 10 −5 M. Proliferation was blocked by 75% at that concentration, and the same relation between the reduction in current and proliferation was seen throughout the concentration range. A similar reduction of Ca currents and proliferation was demonstrated with octanol, a long‐chain alcohol that has recently been reported to block Ca channels. These results suggest a role for Ca channels in the proliferation of MHY206 cells and implicate blockage of these channels as contributing to the antiproliferative activity of RA.