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Effects of diacylglycerols on LLC‐PK 1 renal epithelia: Similarity to phorbol ester tumor promoters
Author(s) -
Mullin James M.,
McGinn Mary T.
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041340306
Subject(s) - diacylglycerol kinase , protein kinase c , diglyceride , phorbol , biochemistry , mannitol , biology , chemistry , biophysics , signal transduction , microbiology and biotechnology , enzyme
As previously shown using phorbol ester tumor promoters (see Mullin and O'Brien: Am. J. Physiol., 251 :C597–C602, 1986), diacylglycerols induce leakiness in LLC‐PK 1 renal epithelial tight junctions. The similarity between phorbol ester and diacylglycerol action includes effects on (1) cell morphology, (2) dome formation, (3) transepithelial resistance and potential difference, (4) transepithelial flux of D‐mannitol, and (5) mitogenesis. Four diacylglycerols have been tested: 1,2‐dioctanoylglycerol; 1,2‐dicaprylglycerol; 1,2‐dioleoylglycerol; and 1‐oleoyl‐2‐acetoyl‐sn‐3‐glycerol. Their relative effectiveness depended upon the phenomenon being observed. Unlike phorbol esters, diacylglycerol effects were reversible within hours at 37°C in the continued presence of diacylglycerol, and effects were more pronounced when cell sheets were exposed to diacylglycerols from the basolateral cell surface. Overall, these findings indicate that previous results with phorbol esters may be attributed to the protein kinase C signal transduction system, and this system may therefore exert a role in transepithelial permeability.