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Stimulation of mono (ADP‐ribosyl)ation by reduced extracellular calcium levels in human fibroblasts
Author(s) -
Duncan Michael R.,
Rankin Patrick R.,
King Robert L.,
Jacobson Myron K.,
Dell'Orco Robert T.
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041340121
Subject(s) - nad+ kinase , extracellular , calcium , fibroblast , biochemistry , stimulation , adp ribosylation , chemistry , microbiology and biotechnology , biosynthesis , biology , enzyme , in vitro , endocrinology , organic chemistry
Lowering extracellular calcium in cultures of human diploid fibroblast‐like cells caused a rapid depletion of NAD pools. This loss of NAD was reversed by restoring extracellular Ca 2+ and was inhibited by 3‐aminobenzamide, an inhibitor of ADP‐ribosyl transfer reactions. The concentrations of 3‐aminobenzamide needed to inhibit the loss of NAD were consistent with those required to inhibit cellular mono(ADP‐ribosyl) rather than poly(ADP‐ribosyl) reactions. Calcium depletion did not inhibit the biosynthesis of NAD. These results suggest that mono(ADP‐ribosyl)ation is involved in the regulation of cellular Ca 2+ levels.