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Activation and proliferation signals in murine macrophages: Stimulation of Na + , K + ‐ATPase activity by hemopoietic growth factors and other agents
Author(s) -
Vairo Gino,
Hamilton John A.
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041340103
Subject(s) - ouabain , stimulation , dna synthesis , haematopoiesis , microbiology and biotechnology , atpase , concanavalin a , colony stimulating factor , biology , intracellular , macrophage , chemistry , biochemistry , endocrinology , sodium , enzyme , dna , in vitro , stem cell , organic chemistry
Abstract Purified colony stimulating factor (CSF‐1) stimulates the Na + , K + ‐ATPase activity of murine bone marrow‐derived macrophages (BMM) and resident peritoneal macrophages (RPM) measured as ouabain‐sensitive 86 Rb + uptake. Similar concentrations of CSF‐1 stimulate the Na + , K + ‐ATPase activity and DNA synthesis in BMM whilst ouabain, a specific inhibitor of the Na + , K + ‐ATPase, also inhibits this CSF‐1‐mediated DNA synthesis. Other purified hemopoietic growth factors, granulocyte‐macrophage CSF (GM‐CSF) and interleukin‐3 (IL‐3), and the tumor promoter, 12‐0‐tetradecanoyl‐phorbol‐13‐acetate (TPA), even though differing in their mitogenic capabilities, are also stimulators of the Na + ,K + ‐ATPase activity in BMM and RPM. The non‐mitogenic agents, lipopolysaccharide (LPS) and Concanavalin A (Con A), are also active. CSF‐1 stimulation of the Na + ,K + ‐ATPase was shown to be dependent on elevation of intracellular Na + via an amiloride sensitive Na + ‐channel, most likely representing Na + /H + exchange activity. Such stimulation of Na + ,K + ‐ATPase activity via activation of the Na + /H + exchange appears to be a necessary but insufficient early macrophage response for subsequent DNA synthesis.