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Quantitation and tissue localization of the cellular retinoic acid‐binding protein
Author(s) -
Eriksson Ulf,
Hansson Eva,
Nordlinder Hans,
Busch Christer,
Sundelin Johan,
Peterson Per A.
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041330308
Subject(s) - retinoic acid , sertoli cell , germinal epithelium , biology , epithelium , binding protein , retinol binding protein , microbiology and biotechnology , retinol , endocrinology , biochemistry , spermatogenesis , vitamin , genetics , gene
The distribution of the cellular retinoic acid‐binding protein (CRABP) in some rat tissues has been determined, and the protein has been localized by immunocytochemical techniques in sections from rat testis. In the testis CRABP was found in the seminiferous tubuli with Sertoli cells and the spermatogonia most intensely stained. All other cells of the germinal epithelium appeared largely devoid of CRABP. By use of an enzyme‐linked immunosorbent assay CRABP was quantitatively estimated in several tissues and the highest levels were found in testis and eye. Comparisons of the tissue levels of CRABP and of the cellular retinol‐binding protein (CRBP) did not reveal any apparent correlation.