z-logo
Premium
Intraclonal diversity of fibrosarcoma cells for the production of macrophage colony‐stimulating factor and granulocyte colony‐stimulating factor
Author(s) -
Sakai Nobuo,
Kubota Masao,
Shikita Mikio,
Yokota Masahiko,
Ando Koichi
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041330228
Subject(s) - colony stimulating factor , biology , granulocyte macrophage colony stimulating factor , fibrosarcoma , cell culture , macrophage colony stimulating factor , macrophage , microbiology and biotechnology , leukocytosis , cloning (programming) , immunology , haematopoiesis , stem cell , cytokine , biochemistry , genetics , in vitro , computer science , programming language
A new cell line was established from fibrosarcoma that had spontaneously developed in a mouse. The cells were maintained growing in cultue for two years and constantly produced both macrophage colony‐stimulating factor (M‐CSF) and granulocyte colony‐stimulating factor (G‐CSF). Cloning of the cells by anchorage‐independent colony formation gave subclones showing the activity of producing M‐CSF and G‐CSF in different proportions, whereas no subclone produced G‐CSF without producing M‐CSF simultaneously. Recloning of the bipotential subclones again gave clonal derivatives producing two types of CSF in various proportions. The observed heterogeneity of the cloned cells seems to be an epigenetic phenomenon, because the cells resumed the G‐CSF producing activity in the absence of cell proliferation. After equilibrium was achieved, all of the subclones produced both M‐CSF and G‐CSF nearly in equal proportions. Tumorigenic and leukocytosis‐inducing activity of the cloned cells was nearly comparable with the activity of the original tumor cells.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here