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Megakaryocyte colony growth‐supporting activities in human plasma: Modification by platelets and platelet membranes
Author(s) -
Yamasaki Kazuo,
Jamal Nazir,
Mann Kenneth G.,
Messner Hans A.
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041330218
Subject(s) - megakaryocyte , platelet , sephadex , membrane , size exclusion chromatography , chemistry , biochemistry , platelet activation , biology , microbiology and biotechnology , immunology , haematopoiesis , enzyme , stem cell
Human megakaryocyte colonies are grown in methylcellulose with platelet‐poor plasma and medium conditioned by phytohemagglutinin‐stimulated leukocytes (PHA‐LCM) as a source of megakaryocyte colony stimulating factor (MEG‐CSF). The megakaryocyte colony growth‐supporting activity in human plasma can be absorbed by intact platelets or degranulated platelet membranes. It was possible to recover the activity by solubilized platelet membrane with cholic acid. Filtration of the solubilized platelet membrane preparations through a Sephadex G‐100 column yielded at least two activity peaks. The molecular weight of these two activities differs from that of the growth‐promoting activity in PHA‐LCM.