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Human breast epithelial cells in serum‐free collagen gel primary culture: Growth, morphological, and immunocytochemical analysis
Author(s) -
Yang Jason,
Balakrishnan Arun,
Hamamoto Susan,
Elias Joel J.,
Rosenau Werner,
Beattie Craig W.,
Das Gupta Tapas K.,
Wellings Sefton R.,
Nandi Satyabrata
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041330205
Subject(s) - myoepithelial cell , staining , immunocytochemistry , biology , epithelium , cell culture , microbiology and biotechnology , pathology , chemistry , endocrinology , immunology , immunohistochemistry , medicine , genetics
Human breast epithelial cells derived from various sources (fibroadenoma, reduction mammoplasty, and mastectomy tissues from premenopausal patients) have been cultured in collagen gel matrix using serum‐free medium. Response to various additives has been analyzed for growth‐promoting effect when added to a basal medium containing insulin, cholera toxin, and BSA. A consistent observation has been the effect of EGF and cortisol in growth stimulation of human breast epithelial cells, while separately, each additive elicited only a small response. Under this condition, employing EGF and cortisol combinations, these cells gave rise to organized colonies consisting of clusters of cells, usually spherical, without any duct‐like extensions. Ultrastructural and immunocytochemical studies, using a panel of monoclonal and polyclonal antibodies, have shown that cell types and features that can be identified in the original breast tissue can also be delineated in the progeny populations. The topographical feature, consisting of lumina surrounded by a single inner layer of epithelial cells and an outer layer of basal/myoepithelial cells, can be re‐created in the collagen gel system starting from small clumps of cells.