Premium
Erythropoiesis in murine long‐term bone‐marrow cell cultures: Dependence on erythropoietin and endogenous production of an erythropoietic stimulating activity
Author(s) -
Oddos Thierry,
NacolLizard Sarab,
Blanchet Jean Paul
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041330109
Subject(s) - erythropoietin , erythropoiesis , bone marrow , stimulation , endocrinology , medicine , cell culture , biology , endogeny , in vitro , microbiology and biotechnology , immunology , anemia , biochemistry , genetics
Erythropoiesis was obtained in murine long‐term bone‐marrow cell cultures (LTBMCs) in the presence of erythropoietin (Epo) when the medium was frequently renewed. The level of the erythropoietic differentiation was shown to be a function of the erythropoietin concentration. In response to Epo addition, an activity which stimulates CFU‐E proliferation in semisolid cultures of fresh bone marrow cells was detected in the LTBMC supernatants. These results suggest that another factor, whose synthesis may be under Epo control, participates in the stimulation of erythropoiesis in vitro.