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Elevation of a potassium current in differentiating human leukemic (HL‐60) cells
Author(s) -
Wieland Steven J.,
Chou Robin H.,
Chen Thelma A.
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041320227
Subject(s) - depolarization , intracellular , patch clamp , chemistry , retinoic acid , phorbol , membrane potential , protein kinase c , microbiology and biotechnology , biophysics , medicine , endocrinology , biology , biochemistry , kinase , receptor , gene
Human promyelocytic leukemia (HL‐60) cells display a novel voltage‐dependent outward current under voltage clamp. This current is present at low levels in the proliferative state and in granulocytes derived from HL‐60 cells which were induced to differentiate with retinoic acid. It is elevated in macrophages derived from HL‐60 cells after exposure to phorbol‐12‐myristate‐13‐acetate (PMA). The current is carried primarily by K + , is blocked by Cs + and by increased intracellular concentrations of Cl − . From a holding potential of −80 mV, significant activation required depolarization to +20 mV membrane potential. Activation was not influenced by intracellular Ca 2+ (1–2 × 10 −6 M). These properties appear to differ significantly from the Ca 2+ ‐activated K + channel and the delayed rectifier. The increase of this voltage‐activated current in differentiation toward the macrophage, but not the granulocyte, suggests that this current is correlated specifically with macrophage differentiation.