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Effects of EGF and calcium on adult parenchymal hepatocyte proliferation
Author(s) -
Eckl Peter M.,
Whitcomb Wendi R.,
Michalopoulos George,
Jirtle Randy L.
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041320225
Subject(s) - hepatocyte , mitotic index , calcium , parenchyma , cell division , mitosis , epidermal growth factor , hepatocyte growth factor , endocrinology , medicine , cell growth , andrology , biology , chemistry , cell , microbiology and biotechnology , in vitro , biochemistry , receptor , botany
Adult rat hepatocytes were grown in serum‐free medium containing 0.05‐4 mM Ca + + and 40 ng/ml EGF. After 48 hours of cultivation the mitotic index and the percentage of second division metaphases were determined. The results demonstrated a maximum proliferation response to EGF at a Ca + + concentration of 0.4 mM. With lower and higher external Ca + + concentrations the fraction of cells undergoing more than one cell division decreased. At lower Ca + + concentrations this decrease appears to result from a reduced viability. In contrast, the low response to EGF at higher Ca + + concentrations—especially in the physiological range—may reflect the influence of Ca + + on the state of hepatocyte differentiation.