Effects of EGF and calcium on adult parenchymal hepatocyte proliferation | Zendy

Eckl Peter M. | Zendy; Whitcomb Wendi R. | Zendy; Michalopoulos George | Zendy; Jirtle Randy L. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Effects of EGF and calcium on adult parenchymal hepatocyte proliferation

Author(s) -

Eckl Peter M.,

Whitcomb Wendi R.,

Michalopoulos George,

Jirtle Randy L.

Publication year - 1987

Publication title -

journal of cellular physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.529

H-Index - 174

eISSN - 1097-4652

pISSN - 0021-9541

DOI - 10.1002/jcp.1041320225

Subject(s) - hepatocyte , mitotic index , calcium , parenchyma , cell division , mitosis , epidermal growth factor , hepatocyte growth factor , endocrinology , medicine , cell growth , andrology , biology , chemistry , cell , microbiology and biotechnology , in vitro , biochemistry , receptor , botany

Adult rat hepatocytes were grown in serum‐free medium containing 0.05‐4 mM Ca + + and 40 ng/ml EGF. After 48 hours of cultivation the mitotic index and the percentage of second division metaphases were determined. The results demonstrated a maximum proliferation response to EGF at a Ca + + concentration of 0.4 mM. With lower and higher external Ca + + concentrations the fraction of cells undergoing more than one cell division decreased. At lower Ca + + concentrations this decrease appears to result from a reduced viability. In contrast, the low response to EGF at higher Ca + + concentrations—especially in the physiological range—may reflect the influence of Ca + + on the state of hepatocyte differentiation.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research