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Calcium and calmodulin in cellular intoxication with Clostridium difficile toxin B
Author(s) -
Caspar Maria,
Florin Inger,
Thelestam Monica
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041320124
Subject(s) - calmodulin , internalization , verapamil , clostridium difficile toxin b , calcium , toxin , trifluoperazine , extracellular , chemistry , clostridium difficile toxin a , calcium channel , cytosol , pharmacology , ionophore , biochemistry , clostridium difficile , microbiology and biotechnology , biology , receptor , antibiotics , enzyme , organic chemistry
In cultured human lung fibroblasts treated with Clostridium difficile toxin B, the development of the cytopathogenic effect was inhibited by the proton ionophore monensin but was not affected by some other ionophores. The calcium channel blockers verapamil and LaCl 3 protected the cells against intoxication, as did the calmodulin antagonists trifluoperazine, amitriptylin, R 24571, and dansylcadaverine. Since these agents could not prevent intoxication when added after toxin internalization was completed, we suggest that calmodulin and uptake of extracellular calcium are needed for the internalization but not for the cytosolic action of the toxin.