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Inhibition by cytochalasin B of DNA synthesis in a thermosensitive anchorage‐independent growth mutant
Author(s) -
Miyashita Kiichi
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041310115
Subject(s) - dna synthesis , cytochalasin b , mutant , cytochalasin d , microbiology and biotechnology , chinese hamster , dactinomycin , protein biosynthesis , biology , transcription (linguistics) , dna , cytochalasin , cell division , cell culture , cell , biochemistry , cycloheximide , cytoskeleton , gene , genetics , linguistics , philosophy
After a shift from a nonpermissive to a permissive temperature, synchronized DNA synthesis and cell division were observed in a cold‐sensitive anchorageindependent growth mutant (cs‐17–25) of Chinese hamster lung cells in Methocel culture. Only 15 min exposure to the permissive temperature was sufficient for induction of DNA synthesis in the cells. A low dose of actinomycin D (0.02 μg/ml) or cytochalasin B (5 μg/ml) was able to inhibit the DNA synthesis when added at an early period after the temperature shift. The inhibitory effects of actinomycin D and cytochalasin B on RNA and protein synthesis were very similar at both temperatures. The degree of multinucleation caused by cytochalasin B was altered quickly depending on the incubation temperature. These results suggest that stimulation of this mutant involves an obligatory actin‐mediated step closely correlated to transcription of early mRNAs.