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Differentiation‐associated decrease in muscarinic receptor sensitivity in human neuroblastoma cells
Author(s) -
Heikkilä Jari E.,
Scott Ian G.,
Suominen Liisa A.,
Åkerman Karl E. O.
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041300122
Subject(s) - agonist , muscarinic acetylcholine receptor , intracellular , endocrinology , stimulation , cytosol , chemistry , muscarinic agonist , neuroblastoma , receptor , cell culture , medicine , antagonist , biophysics , biology , biochemistry , enzyme , genetics
Muscarinic receptor‐linked increases in intracellular free Ca 2+ as measured with quin‐2 and Ca 2+ release from monolayers of cells have been measured in the human neuroblastoma cell line SH‐SY5Y. Induction of differentiation with the phorbol ester 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) leads to a decrease in the sensitivity of the cells to low concentrations of agonists with respect to the induced increase in cytosolic free Ca 2+ and stimulation of Ca 2+ efflux. No decrease in agonist binding affinity was observed when the displacement of a labelled antagonist, 3 H‐NMS, by a non‐labelled agonist was studied.