Premium
Characterisation of stroma‐dependent blast colony‐forming cells in human marrow
Author(s) -
Gordon M. Y.,
Dowding C. R.,
Riley G. P.,
Greaves M. F.
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041300121
Subject(s) - stromal cell , progenitor cell , bone marrow , biology , population , colony forming unit , microbiology and biotechnology , precursor cell , stem cell , immunology , stroma , cell , cancer research , medicine , genetics , immunohistochemistry , environmental health , bacteria
Human bone marrow contains a population of haemopoietic progenitor cells that can be distinguished by their ability to adhere to preformed stromal layers cultured in the presence of methylprednisolon [MP + ] and form blast cell colonies. The stromal layers function in the colony assay after they have been heavily irradiated but not after they have been passaged. The binding of the progenitor cells to the stromal cells is complete after 2 hours of coincubation, and stromal layers of 9.6 cm 2 can provide adhesion sites for at least 2,000 blast colony‐forming cells. The blast colony‐forming cells were shown by micromanipulation to self‐renew as well as to give rise to multipotential and lineage‐committed colony‐forming progenitor cells.