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Properties of colony‐stimulating factors produced by macrophage cell lines and hybrid cells
Author(s) -
Ohki Kazunori,
Nagayama Ariaki
Publication year - 1987
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041300111
Subject(s) - colony stimulating factor , biology , cell culture , microbiology and biotechnology , granulocyte macrophage colony stimulating factor , chinese hamster , macrophage colony stimulating factor , hamster , macrophage , lipopolysaccharide , fibroblast , zymosan , cytokine , immunology , haematopoiesis , in vitro , biochemistry , genetics , stem cell
Colony‐stimulating factors (CSFs) produced by two simian virus 40(SV40) transformed macrophage cell lines ( BAM1 and BAM3 ), and three hybrids (HM3‐11, HM3‐12, and HM3‐14) derived from fusion between BAM3 and a Chinese hamster cell line (hs222‐16) were examined. HM3‐11 and HM3‐14 produce two molecular species of CSF, which are not found in the conditionec media from cultures of BAM1 and BAM3 or lipopolysaccharide (LPS), phorbolmyristate‐acetate (PMA), and zymosan‐stimulated BAM3. HM3‐12, which is classified into another group in terms of CSF secretion, does not produce these two CSFs. On the basis of various criteria, one of these CSF species ( peak 1‐CSF ) was characterized as a macrophage‐colony‐stimulating factor (M‐CSF). The other CSF (peak 2‐CSF) induced a group of bone marrow cells in granulocytes and macrophages as well as growth of a mast cell line, IC2. This CSF has an apparent molecular weight of 18,000, estimated by SDS‐polyacrylamide gel electrophoresis. Unlike interleukin 3 (IL3) from WEHI‐3 cells, the growth factor activity of peak 2‐CSF binds to DEAE‐Sephacel. Thus, peak 2‐CSF is similar to a granuclocyte‐macrophage colony‐stimulating factor (GM‐CSF) rather than to IL3. The anti L cell CSF serum does not inhibit the CSF activity in Chinese hamster fibroblast conditioned medium, and the IC2 cells do not respond to Chinese hamster lung conditioned medium (CHLCM), suggesting that peak 1‐ and peak 2‐CSF are of mouse origin.