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Both normal and tumor cells produce basic fibroblast growth factor
Author(s) -
Moscatelli David,
Presta Marco,
JosephSilverstein Jacquelyn,
Rifkin Daniel B.
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041290220
Subject(s) - fibroblast growth factor , basic fibroblast growth factor , collagenase , autocrine signalling , biology , angiogenesis , microbiology and biotechnology , cell culture , fibroblast , chemistry , growth factor , in vitro , biochemistry , cancer research , receptor , enzyme , genetics
We have previously purified from human placenta a basic fibroblast growth factor (FGF)‐like molecule which stimulates the production of plasminogen activator (PA) and collagenase, induces DNA synthesis, produces an increase in motility in cultured bovine capillary endothelial (BCE) cells, and induces angiogenesis in vivo. The ability of basic FGF to stimulate PA production in BCE cells was used as an assay for the presence of basic FGF‐like molecules in extracts of both normal and tumor‐derived cultured cells. The identity of the PA‐stimulatory activity with basic FGF was confirmed by its high affinity for heparin and by its cross‐reactivity with antibodies to human placental basic FGF. Basic FGF‐like molecules were identified in eight of ten cell lines tested, and the amount of FGF‐like activity present in these cells bore no relation to their origin from normal or tumor tissue. The test cells, BCE cells, had one of the highest levels of FGF‐like activity, suggesting that it may have an autocrine role in these cells.