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Spontaneous differentiation of murine teratocarcinoma stem cells at low temperature
Author(s) -
Cremisi Chantal,
Duprey Philippe
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041290215
Subject(s) - teratocarcinoma , cytokeratin , microbiology and biotechnology , in vitro , biology , messenger rna , cellular differentiation , actin , cell culture , stem cell , cytoskeleton , cell , gene , immunology , genetics , immunohistochemistry
Embryonal carcinoma cells differentiate in vitro after treatment with different drugs or under certain culture conditions. We show here that at 31°C PCC‐aza‐1 cells differentiated spontaneously into cells with characteristics resembling those of epithelial cells. With this temperature, we observed morphological changes; reorganization of the cytoskeleton with polymerization of actin cables, tight junctions, and cytokeratin endo A messenger RNA and protein expression The cells also became totally permissive to Py virus but remained resistant to SV40 infection. This new pathway of differentiation has the advantage of being reversible at 37°C.