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Effects of transformation on the expression of laminin and fibronectin by neural cells
Author(s) -
Giotta Gregory J.,
Newby Ron,
Oliver Noelynn
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041290107
Subject(s) - fibronectin , laminin , rous sarcoma virus , transformation (genetics) , cell culture , microbiology and biotechnology , immunofluorescence , biology , neural cell , cell , extracellular matrix , biochemistry , immunology , antibody , genetics , virus , gene
We have studied laminin and fibronectin expression in a collection of rat cerebellar neural cell lines transformed with a mutant of Rous sarcoma virus which is temperature sensitive for transformation. We show that regardless of their neuronal or glial properties the cell lines produce both laminin and fibronectin. Laminin is expressed in similar amounts in cell lines grown at either the permissive or nonpermissive temperature for transformation, while fibronectin is generally expressed at higher levels in cells kept at the nonpermissive temperature. To provide further evidence that neural cells can produce laminin and fibronectin, double label immunofluorescence studies were conducted on primary cerebellar cultures. Both laminin and fibronectin were found to be present in the primary culture, and laminin was found to be associated with a subpopulation of astrocytes.