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Mc3T3‐G2/PA6 preadipoctyes support in vitro proliferation of hemopoietic stem cells through a mechanism different from that of interleukin 3
Author(s) -
Kodama HiroAki,
Hagiwara Hajime,
Sudo Hiroko,
Amagai Yuji,
Yokota Takashi,
Arai Naoko,
Kitamura Yukihiko
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041290104
Subject(s) - haematopoiesis , interleukin 3 , bone marrow , stem cell , stem cell factor , microbiology and biotechnology , biology , in vitro , interleukin 33 , immunology , interleukin , chemistry , cytokine , interleukin 21 , cytotoxic t cell , biochemistry
Abstract Both MC3T3‐G2/PA6 preadipocytes and interleukin 3 (IL 3) can support in vitro proliferation of mouse hemopoietic stem cells (CFU‐S). We examined whether MC3T3‐G2/PA6 cells produce IL 3 and whether a common mechanism might underlie the action of both of these agents. We used cultured mast cells, DA‐1 cells, and FDC‐P2 cells as the targets of IL 3 and conditioned medium (CM) of WEHI‐3 cells as a source of IL 3. MC3T3‐C2/PA6 CM did not support the growth of the above cells. IL 3 mRNA was not detected in the preadipocytes. Since CM obtained from the cocultures of bone marrow cells and MC3T3‐G2/ PA6 cells did not have a significant effect on the growth of the IL 3‐dependent cells, none of the bone marrow cells seem to produce IL 3 under the influence of the preadipocytes. When the factor‐dependent cells were cocultured with MC3T3‐G2/PA6 cells, the former did not survive, whereas mast cells and DA‐1 cells intimately associated with the preadipocytes. Even when bone marrow cells, mast cells, and MC3T3‐G2/PA6 cells were cocultured, the number of CFU‐S increased, but not that of mast cells. These results seem to exclude the possibility of the action of IL 3 in the microenvironment provided by MC3T3‐G2/PA6 preadipocytes.