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Specific inhibition of endothelial cell proliferation by isolated endothelial plasma membranes
Author(s) -
Teitel Jerome M.
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041280227
Subject(s) - differential centrifugation , cell growth , endothelial stem cell , membrane , hemocytometer , cell , biology , cell culture , centrifugation , chemistry , biochemistry , microbiology and biotechnology , biophysics , in vitro , genetics
Cultures of human vascular endothelial cells were used to study the phenomenon of density‐dependent inhibition of cell growth. Endothelial cells were disrupted by nitrogen cavitation, and a plasma membrane‐enriched fraction was prepared by differential centrifugation followed in some cases by sucrose density gradient fractionation. Membrane suspension was added to lowdensity early‐passage endothelial cultures grown in microwells. Hemocytometer cell counts and 6 hr 3 H‐thymidine pulses were performed in triplicate wells at varying intervals. Plasma membranes suppressed cell proliferation in a reversible, dose‐dependent fashion. Increasing the ambient concentration of endothelial cell growth factor did not alter the inhibitory effect. The antiproliferative effect was sensitive to heat and trypsin and to incubation with 0.1 M sodium carbonate, pH 11.5. Membrane vesicles selectively derived from the apical cell surface also suppressed proliferation. This phenomenon showed at least some specificity for cell type and species in both human and bovine models. Therefore, cell‐cell contact is capable of regulating endothelial cell proliferation in vitro despite the presence of available growth surfaces and of optimally supportive culture medium.