Premium
Characterization of RNA polymerase type II from human term placenta
Author(s) -
Freund Erwin,
McGuire Peter M.
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041270312
Subject(s) - polyacrylamide gel electrophoresis , chemistry , divalent , placenta , purine , enzyme , polymerase , biochemistry , nucleotide , rna , microbiology and biotechnology , biology , gene , fetus , pregnancy , organic chemistry , genetics
RNA polymerase type II from human term placenta has been isolated and characterized with respect to its template, ammonium sulfate, divalent cation, and buffer preferences. In addition, the apparent Michaelis constants for AMP amd UMP incorporation have been determined. The enzyme was also analyzed by native and denaturing polyacrylamide gel electrophoresis, and evidence is presented that a single polypeptide is radiolabeled with azido purine nucleoside triphosphate photoprobes.