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Retinoic acid effect on cyclic AMP‐dependent protein kinases in embryonal carcinoma cells: Studies with differentiation‐defective sublines
Author(s) -
Plet Ariane,
Gerbaud Pascale,
Sherman Michael I.,
Anderson Wayne B.,
Brion Daniele Evain
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041270223
Subject(s) - retinoic acid , protein kinase a , cytosol , retinoic acid receptor , kinase , embryonal carcinoma , biology , cellular differentiation , microbiology and biotechnology , biochemistry , chemistry , enzyme , gene
Retinoic acid induces the differentiation of PCC4.aza 1R and Nulli‐SCC1 embryonal carcinoma (EC) cells. In response to retinoic acid treatment, the levels of cyclic AMP (cAMP)‐dependent protein kinases are enhanced in the plasma membrane within 17 hours and in the cytosol fractions of these cells within 2 to 3 days, as determined by phosphotransferase activity and by 8‐azido‐cyclic [ 32 P]AMP binding to the RI and RII regulatory subunits. PCC4 (RA) − 1 and Nulli (RA) − 1 are mutant EC lines that fail to differentiate in response to retinoic acid. The former line, but not the latter, lacks cellular retinoic acid‐binding protein (cRABP). Basal levels of cAMP‐dependent protein kinase activities are elevated in PCC4 (RA) − 1 cells. When these cells are treated with retinoic acid, neither cAMP‐dependent protein kinase activities nor cAMP binding activities are enhanced; rather, there is a decrease in cytosolic kinase activity and RI subunit. On the other hand, Nulli (RA) − 1 cells exhibit increases both in cAMP‐dependent protein kinase activities and cAMP binding in response to retinoic acid. These results raise the possibility that cRABP mediates the enhancement of regulatory and catalytic subunits of cAMP‐dependent protein kinases in both the membrane and the cytosolic fractions of the teratocarcinoma cells. There also might be some effects of retinoic acid on the cAMP‐dependent protein kinase that are unrelated to differentiation and to the presence of cRABP.