Premium
Isolation of a human stromal cell strain secreting hemopoietic growth factors
Author(s) -
Tsai Schickwann,
Emerson Stephen G.,
Sieff Colin A.,
Nathan David G.
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041270117
Subject(s) - haematopoiesis , progenitor cell , stromal cell , biology , bone marrow , macrophage , colony stimulating factor , microbiology and biotechnology , granulocyte , immunology , in vitro , stem cell , biochemistry , cancer research
A diploid fibroblastoid cell strain, termed “ST‐1,” has been established from a long‐term liquid culture of human fetal liver cells. ST‐1 cells are nonphagocytic, nonspecific esterase negative and do not possess factor VIII‐related antigen but stain with antibodies specific for fibronectin and type I collagen. The ST‐1 cells produce nondialyzable hemopoietic growth factors capable of stimulating the development of erythroid bursts, mixed granulocyte‐macrophage colonies, pure granulocyte colonies, and pure macrophage colonies. These factors are active on both human fetal liver and human adult bone marrow progenitors. When liquid cultures of human fetal liver hemopoietic progenitors are established with a preformed monolayer of ST‐1 cells, the yields of nonadherent cells, erythroid progenitors, and myeloid progenitors are greatly increased. These studies demonstrate that the fibroblastoid ST‐1 cells support hemopoiesis in vitro and may be a critical element in the stromal microenvironment in vivo.