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Effects of hypoxia and hyperoxia on proteoglycan production by bovine pulmonary artery endothelial cells
Author(s) -
Humphries Donald E.,
Lee SheuLing,
Fanburg Barry L.,
Silbert Jeremiah E.
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041260214
Subject(s) - proteoglycan , hyperoxia , oxygen tension , hypoxia (environmental) , chondroitin sulfate , glycosaminoglycan , chemistry , endothelium , biochemistry , endothelial stem cell , cell culture , microbiology and biotechnology , oxygen , biology , endocrinology , extracellular matrix , in vitro , organic chemistry , genetics
Bovine pulmonary artery endothelial cells in culture were used to assess the influence of oxygen tension on proteoglycan synthesis. Cells exposed to 3% O 2 (hypoxia) for 72 h and then labeled with [ 35 S]sulfate for 5 h accumulated significantly less [ 35 S]proteoglycan in medium than cells exposed to 20% O 2 (control). This decrease was due primarily to a reduction in heparan sulfate. Cells exposed to 80% O 2 (hyperoxia) for 72 h secreted slightly more [ 35 S]proteoglycan into medium than controls. Greater accumulation of chondroitin sulfate was responsible for the increase. The amount of cell‐associated proteoglycan did not change significantly in cells cultured in 3% or 80% O 2 as compared with control cells cultured in 20% O 2 . Proteoglycans produced by hypoxia‐ or hyperoxia‐treated cells were found to be similar in size to proteoglycans produced by cells cultured at 20% O 2 . Glycosaminoglycan sulfation, as measured by ion‐exchange chromatography, did not appear to change with varying oxygen tensions. Our results demonstrate that production of proteoglycans secreted by endothelial cells in culture is sensitive to oxygen tension.