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Reduced pinosome formation in a cell‐fusion‐impaired mutant of mouse lymphocytic cell line L5178Y
Author(s) -
Pauw Peter G.,
Biagi Karen G.,
Stadler Joan K.
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041260213
Subject(s) - internalization , mutant , cell fusion , intracellular , extracellular , microbiology and biotechnology , cell , polyethylene glycol , fusion , sucrose , peg ratio , cell culture , cell membrane , chemistry , lipid bilayer fusion , biophysics , biology , membrane , biochemistry , genetics , linguistics , philosophy , finance , economics , gene
Accumulation and release of a fluid‐phase marker ([ 14 C]‐sucrose) were studied in a subline of the mouse lymphocytic cell line L5178Y and in a polyethylene glycol‐resistant, intercellular‐fusion‐impaired mutant of this line. The mutant was found to accumulate [ 14 C]‐sucrose at a significantly slower rate than the parent. Analysis of release of preloaded label shows that the reduced rate of accumulation is due to a correspondingly low level of internalization, i.e., pinosome formation, rather than to a reduction in delivery of label to lysosomes. Cell‐to‐cell fusion and pinosome formation both involve a fusion event initiated at the extracellular surface of the plasma membrane, and we propose that the coordinate reduction in both processes suggests that they are mechanistically related.