z-logo
Premium
A direct correlation between hyperthermia‐induced membrane blebbing and survival in synchronous G 1 CHO cells
Author(s) -
Borrelli M. J.,
Wong R. S. L.,
Dewey W. C.
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041260206
Subject(s) - organelle , electron microscope , membrane , cell , hyperthermia , cell membrane , biophysics , microbiology and biotechnology , chinese hamster ovary cell , cell culture , biology , chemistry , biochemistry , optics , genetics , physics , paleontology
Heating synchronous G 1 cells at 45.5°C for 3–20 min induced varying degrees of membrane blebbing ranging from nonblebbed cells indistinguishable from control cells to those with blebs larger than the cell itself. Both the proportion of cells exhibiting blebbing and the mean diameter of the blebs increased with heating duration. Scoring individual cells for both blebbing and colony formation demonstrated that cells with blebs larger than 50% of the cell diameter did not survive to form colonies. Electron microscopy showed that all subcellular organelles, save the ribosomes, were absent from the membrane blebs. Freeze fracture replicas revealed no changes in membrane ultra‐structure, except on some 15% of the blebs that contained bald patches devoid of membrane particles.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here