Premium
Effects of maturational agents on expression and secretion of two partially characterized high molecular weight milk‐related glycoproteins in MCF‐7 breast carcinoma cells
Author(s) -
Abe Miyako,
Kufe Donald
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041260117
Subject(s) - antigen , microbiology and biotechnology , biology , monoclonal antibody , breast carcinoma , cell culture , tumor antigen , glycoprotein , cycloheximide , antibody , biochemistry , immunology , cancer , breast cancer , in vitro , cytotoxic t cell , protein biosynthesis , genetics
We have previously defined a human mammary epithelial antigen using a murine monoclonal antibody (MAb), designated DF3, prepared against a membrane‐enriched fraction of a human breast carcinoma. MAb DF3 detects a cell surface antigen with a molecular weight (mw) of approximately 300 Kd and a higher mw species also detectable in human milk. These findings and the demonstration that butyric acid (BA) increases DF3 antigen expression suggested that MAb DF3 reacts with a differentiation antigen detectable in human breast carcinoma cells. The results of the present study demonstrate that MAb DF3 reacts with two mucin‐like high mw glycoproteins (330 and 450 Kd) present in MCF‐7 breast carcinoma cells. The results also demonstrate that the intracellular content and secretion of DF3 antigen is increased by 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) and 1‐β‐D‐arabinofuranosylcytosine (ara‐C). Other known inducers of differentiation including retinoic acid (RA), hexamethylene bisacetamide (HMBA), 1,25‐dihydroxy vitamin D3 (1,25(OH) 2 D 3 ) and certain polar solvents decrease DF3 antigen expression. Furthermore, the results demonstrate that DF3 antigen is secreted and that the extent coincides with changes in intracellular content. Finally, actinomycin D and cycloheximide inhibit the increases in DF3 antigen expression following TPA treatment thus suggesting that newly synthesized RNA and protein are required for induction of this antigen. Thus, the monitoring of DF3 antigen expression may provide a marker for studying maturation of human breast cancer cells.