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Hydrogen peroxide toxicity may be enhanced by heat shock gene induction in Drosophila
Author(s) -
Love Jack D.,
Vivino Alfred A.,
Minton Kenneth W.
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041260109
Subject(s) - hydrogen peroxide , toxicity , heat shock protein , hsp70 , drosophila melanogaster , microbiology and biotechnology , chemistry , heat shock , gene expression , gene , biophysics , biochemistry , biology , organic chemistry
Recent evidence suggests that low dose exposure of cells to hydrogen peroxide and/or induction of heat shock protein (HSP) synthesis will render cells resistant to the lethal effects of a subsequent high dose hydrogen peroxide stress. We explored this possibility in the Drosophila melanogaster Schneider tissue culture line 2. It was found that chronic low dose exposure (1 mM H 2 O 2 for 3 days) resulted in marked potentiation of the toxic effects of a subsequent high dose exposure (50 mM H 2 O 2 for 1 h), as assessed by impairment of uridine incorporation and cell proliferation. Cells preexposed to low dose H 2 O 2 exhibited enhanced heat shock gene transcription upon exposure to high dose H 2 O 2 , as compared to cells that did not receive low dose preexposure. Transcriptional induction of the heat shock genes by a mild non‐toxic heat shock resulted in marked enhancement of the anti‐proliferative effects of a subsequent H 2 O 2 exposure. Thus, low dose hydrogen peroxide exposure or mild heating results in subsequent enhancement of high dose hydrogen peroxide toxicity; this effect correlates with enhanced heat shock gene expression. Possible mechanisms are discussed.