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Endocytosis in sickle erythrocytes: A mechanism for elevated intracellular Ca 2+ levels
Author(s) -
Rubin Elizabeth,
Schlegel Robert A.,
Williamson Patrick
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041260108
Subject(s) - endocytosis , intracellular , mechanism (biology) , chemistry , microbiology and biotechnology , biophysics , biology , cell , biochemistry , philosophy , epistemology
Staining of sickle cells with the fluorescent probes chlortetracycline (a Ca 2+ probe) and diindocarbocyanine (a general membrane probe) revealed the presence of Ca 2+ ‐containing vesicles which are not found in normal erythrocytes. These vesicles increase in number upon deoxygenation, and are apparently formed by endocytosis, as judged by the use of the extracellular fluorescent probe lucifer yellow. The presence of vesicles is not restricted to any particular morphological or density class of cells in the general population.