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Transformation of mouse bone marrow cells by transfection with a human oncogene related to c‐myc is associated with the endogenous production of macrophage colony stimulating factor 1
Author(s) -
Sklar Marshall D.,
Tereba Allan,
Chen Ben D.M.,
Walker William S.
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041250307
Subject(s) - biology , bone marrow , clone (java method) , transfection , microbiology and biotechnology , cell culture , macrophage , macrophage colony stimulating factor , mononuclear phagocyte system , receptor , stem cell factor , growth factor , progenitor cell , in vitro , immunology , stem cell , gene , biochemistry , genetics
We recently derived a series of transformed cell lines by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with a newly described human gene, R‐myc, which has homology to the c‐myc oncogene. In this report, we show that these lines share some features characteristic of cells of the mononuclear phagocyte lineage. Specifically, all cell lines had macrophage‐or monocytelike morphology, contained nonspecific esterase, were phagocytic for latex beads, secreted lysozyme, bore the Mac‐1 antigen, and contained a minority of cells with Fc receptors. However, only a single monocytelike clone had appreciable numbers of cells which bore complement receptor 1, and none were phagocytic for antibody or complementcoated particles, or constitutively secreted Interleukin‐1. All these cell lines secreted a growth factor capable of supporting the in vitro proliferation of bone marrow macrophages. Radiommunoassay and receptor binding studies indicate that this factor is colony stimulating factor 1.