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Differential effects of the tumor promoter phorbol‐12‐myristate‐13‐acetate on the morphological and biochemical differentiation of N‐18 mouse neuroblastoma cells
Author(s) -
Liu Alice Y.C.,
Chen Kuang Yu
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041250305
Subject(s) - phorbol , neuroblastoma , cellular differentiation , phorbol ester , microbiology and biotechnology , tetradecanoylphorbol acetate , biology , cell culture , chemistry , protein kinase c , biochemistry , enzyme , gene , genetics
The phorbol ester tumor promoter phorbol‐12‐myristate‐13‐acetate (PMA) was found to have differential inhibitory effects on the expression of morphological and biochemical differentiation of N‐18 mouse neuroblastoma cells. PMA completely inhibited neurite extension and associated growth characteristics and partially inhibited the increased expression of R 1 cAMP‐binding protein; PMA had no effect on the induction of acetylcholinesterase activity in cells prompted to differentiate either by treatment with 1 mM dibutyryl cAMP or by serum deprivation. 4‐α‐Phorbol‐12, 13‐didecanoate, an inactive analogue of phorbol ester tumor promoter, was without effect. The implications of these findings concerning the mechanism of action of phorbol ester tumor promoters in the control of cell differentiation are discussed.