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Cell cycle phase‐dependent induction of ornithine decarboxylase‐antizyme
Author(s) -
Linden Margareta,
Anehus Siw,
Långström Eva,
Baldetorp Bo,
Heby Olle
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041250215
Subject(s) - ornithine decarboxylase antizyme , ornithine decarboxylase , microbiology and biotechnology , chemistry , biology , biochemistry , enzyme
The activites of ornithine decarboxylase (ODC) and ODC inhibitory protein (ODC‐antizyme) were studied in Ehrlich ascites tumor cells, separated according to their position in the cell cycle by centrifugal elutriation. Release and/or synthesis of ODC‐antizyme was induced by putrescine treatment. Each mouse received an intraperitoneal injection of 25 μmoles of putrescine at 0, 1, 2, and 3 hr after tumor transplantation. Tumor cells obtained from putrescine‐treated and control mice at 4 hr after transplantation were separated into fractions representing all phases of the cell cycle. The cell cycle distribution of the tumor cells in each fraction was determined by flow cytometry. In control tumor cells the ODC activity exhibited two maxima; inlate‐G1/early‐S and in late‐S/G2. A marked decrease in ODC activity was observed in mid‐S phase. This decrease coincided with maximum ODC‐antizyme activity (revealed by putrescine treatment), suggesting that ODC‐antizyme is involved in the regulation of ODC activity during the cell cycle.