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Differential induction of glucose‐regulated and heat shock proteins: Effects of pH and sulfhydryl‐reducing agents on chicken embryo cells
Author(s) -
Whelan Sandra A.,
Hightower Lawrence E.
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041250212
Subject(s) - cycloheximide , extracellular , heat shock protein , inducer , embryo , intracellular , microbiology and biotechnology , biochemistry , protein biosynthesis , chemistry , biology , gene
Glucose‐regulated and heat shock proteins are two subsets of eukaryotic stress proteins that can be induced differentially, simultaneously, and reciprocally. Two new inducers, low extracellular pH and 2‐mercaptoethanol, that stimulate chicken embryo cells to synthesize glucose‐regulated proteins rapidly were found. Two classes of cellular targets for mercaptoethanol were defined operationally, one dependent on and the other independent of protein synthesis. A new inducer of heat shock proteins, high extracellular pH, was found as well. Inductions by low and high extracellular pH were inhibited by actinomycin D but were insensitive to cycloheximide. Inductions of glucose‐regulated and heat shock proteins are discussed in terms of changes in intracellular pH and sulfhydryl oxidation states.