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BrdU‐Hoechst flow cytometry reveals regulation of human lymphocyte growth by donor‐age–related growth fraction and transition rate
Author(s) -
Kubbies M.,
Schindler D.,
Hoehn H.,
Rabinovitch P. S.
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041250209
Subject(s) - flow cytometry , cell cycle , biology , stimulation , kinetics , lymphocyte , compartment (ship) , cell , cell growth , microbiology and biotechnology , andrology , chemistry , immunology , endocrinology , biochemistry , medicine , oceanography , physics , quantum mechanics , geology
An improved BrdU‐Hoechst flow assay was applied to cell kinetic studies of human lymphocyte cultures during a 24–96 hr interval after PHA stimulation. The assay shows that the duration of the initial lag phase and the proportions of noncycling cells increase as a function of donor age, whereas the rates of transition from each cell cycle compartment to the next decrease. Cell cycle arrest occurs in the first S and G2 phase after stimulation of lymphocytes from a 75‐year‐old donor but not from younger donors. The data are consistent with several models of cell cycle kinetics, so long as these models are modified to include a fraction of noncycling cells in each cell cycle compartment.