BrdU‐Hoechst flow cytometry reveals regulation of human lymphocyte growth by donor‐age–related growth fraction and transition rate | Zendy

Kubbies M. | Zendy; Schindler D. | Zendy; Hoehn H. | Zendy; Rabinovitch P. S. | Zendy

Premium

BrdU‐Hoechst flow cytometry reveals regulation of human lymphocyte growth by donor‐age–related growth fraction and transition rate

Author(s) -

Kubbies M.,

Schindler D.,

Hoehn H.,

Rabinovitch P. S.

Publication year - 1985

Publication title -

journal of cellular physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.529

H-Index - 174

eISSN - 1097-4652

pISSN - 0021-9541

DOI - 10.1002/jcp.1041250209

Subject(s) - flow cytometry , cell cycle , biology , stimulation , kinetics , lymphocyte , compartment (ship) , cell , cell growth , microbiology and biotechnology , andrology , chemistry , immunology , endocrinology , biochemistry , medicine , oceanography , physics , quantum mechanics , geology

An improved BrdU‐Hoechst flow assay was applied to cell kinetic studies of human lymphocyte cultures during a 24–96 hr interval after PHA stimulation. The assay shows that the duration of the initial lag phase and the proportions of noncycling cells increase as a function of donor age, whereas the rates of transition from each cell cycle compartment to the next decrease. Cell cycle arrest occurs in the first S and G2 phase after stimulation of lymphocytes from a 75‐year‐old donor but not from younger donors. The data are consistent with several models of cell cycle kinetics, so long as these models are modified to include a fraction of noncycling cells in each cell cycle compartment.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research