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Continuous growth and phosphatidylcholine synthesis of rat hepatoma cells in choline‐deprived chemically defined medium
Author(s) -
Yamamoto Katsuhiko,
Niwa Akira,
Yasumura Yosihiro
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041250112
Subject(s) - phosphatidylethanolamine , phosphatidylcholine , choline , phosphatidylserine , phospholipid , biochemistry , phosphocholine , methionine , cell culture , chemistry , biology , membrane , amino acid , genetics
A choline non‐required cell line was established from a rat hepatoma cell line. The line designated R‐Y121B·cho was able to grow in choline‐deprived medium without serum and lipid. Choline is necessary for the biosynthesis of phosphatidylcholine, which is a main component of cell membranes. Phosphatidylcholine can be synthesized by the methylation of phosphatidyletha‐nolamine in liver cells. Phospholipid composition and incorporation of radiolabeled serine into phosphatidylserine or phosphatidylethanolamine were quite similar in R‐Y121B cho and its parental cells. However R‐Y121B cho cells had higher phosphatidylcholine synthesis activity from radiolabeled methionine than parental cells. These results indicate that choline requirement of mammalian cells depends on the activity of phosphatidylethanolamine methyltransferase.