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Cell growth and differentiation in vitro in mouse macrophages transformed by a tsA mutant of simian virus 40. III. Large T antigen level and cell proliferation and survival in an SV40 tsA 640‐transformed macrophage line
Author(s) -
Tanigawa Takahiko,
Shimura Hideo,
Yamada Koji,
Okuda Atsuyuki,
Takayama Hisao,
Takagi Atsushi,
Tanaka Yoshinori,
Kimura Genki
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041250104
Subject(s) - mutant , biology , in vitro , virus , simian , cell growth , antigen , virology , microbiology and biotechnology , cell , cell culture , immunology , genetics , gene
The levels of simian virus 40 (SV40) large T antigen in a tsA ‐transformed mouse macrophage line at the permissive (33°C) and the nonpermissive (30°C) temperature were examined by immunofluorescence, sodium dodecylsulfate‐polyacrylamide gel electrophoresis, complement fixation, and enzyme‐linked immunosorbent assay. When the cells were confluent and rested at 33°C, and then were shifted to 39°C, the amount of large T antigen per cell decreased, and most cells survived and remained phagocytic. When the cells were proliferating at 33°C, and then were shifted to 39°C, the cells died with only a small reduction in the amount of large T antigen. Therefore, the physiological state of the cells may determine the survival of cells by affecting the level of large T antigen after exposure to 39°C. The confluent cells may be rested with a concomitant decrease of large T antigen. The proliferating cells may not survive in the presence of a relatively high level of functionally defective large T antigen at 39°C.