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DNA damage production in CHO cells at elevated temperatures
Author(s) -
Warters Raymond L.,
Brizgys Lucy M.,
AxtellBartlett Joann
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041240318
Subject(s) - chinese hamster ovary cell , sucrose , dna , denaturation (fissile materials) , chemistry , nuclear dna , hamster , microbiology and biotechnology , nucleus , biochemistry , mole , activation energy , biophysics , biology , nuclear chemistry , gene , organic chemistry , receptor , mitochondrial dna
Induction of DNA lesions in the nucleus of Chinese hamster ovary (CHO) cells was observed at hyperthermic temperatures using the alkaline filter elution and the alkaline sucrose gradient sedimentation methods. These lesions were observed principally at temperatures greater than 45°C with an activation energy of 140 kcal/mole. On alkaline sucrose gradients the cell genome was reduced to a 140 S or 2 × 10 8 dalton subunit of DNA independent of increasing exposure time at temperatures above 45°C. The large thermal activation energy and the limited DNA size reduction suggest the possible involvement of thermal denaturation of a nuclear polypeptide in the production of these nuclear lesions.