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1α, 25‐Dihydroxyvitamin D 3 specific regulation of growth, morphology, and fibronectin in a human osteosarcoma cell line
Author(s) -
Franceschi Renny T.,
James Wilbur M.,
Zerlauth Gerold
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041230316
Subject(s) - fibronectin , cell culture , endocrinology , alkaline phosphatase , medicine , cell growth , metabolite , biology , vitamin d and neurology , calcitriol receptor , cell , osteosarcoma , receptor , glycoprotein , calcitriol , microbiology and biotechnology , biochemistry , enzyme , cancer research , genetics
The ability of the hormonally active vitamin D metabolite, lα,25‐dihydroxyvitamin D 3 , to affect cell growth, morphology and fibronectin production has been examined using the MG‐63 human osteosarcoma cell line. Hormone treatment reduced cell growth rate, saturation density and [ 3 H]thymidine incorporation. Inhibition was specific for lα,25‐dihydroxyvitamin D 3 relative to other vitamin D metabolites (lα,25‐dihydroxyvitamin D 3 > 25‐dihydroxyvitamin D 3 > 24R,25‐dihydroxyvitamin D 3 D 3 ), antagonized by high concentrations of serum and readily reversed by removal of lα,25‐dihydroxyvitamin D 3 from the culture medium. Hormone treatment also increased cell associated alkaline phosphatase activity up to twofold and altered morphology such that treated cells were more spread out on the culture dish and contained more cytoplasmic processes. Significantly, lα,25‐dihydroxyvitamin D 3 increased cellular and medium concentrations of fibronectin, a glycoprotein known to be involved in cellular adhesiveness. MG‐63 cells contain a specific lα,25‐dihydroxyvitamin D 3 receptor which may mediate these responses.