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Butyrate stimulates fibronectin synthesis in cultured rabbit cornea
Author(s) -
Tanaka Hitoshi,
Nishida Teruo
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041230206
Subject(s) - fibronectin , butyrate , chemistry , stimulation , cornea , butyric acid , wound healing , biochemistry , microbiology and biotechnology , pharmacology , biology , endocrinology , immunology , cell , neuroscience , fermentation
To understand corneal wound healing, in which fibronectin plays an important role, we investigated the regulatory mechanism of fibronectin synthesis in cultured rabbit corneal blocks in situ. The amount of fibronectin was determined by enzyme‐linked immunosorbent assay. We found that butyrate stimulated fibronectin synthesis in a dose‐response fashion, and that butyrate had a greater stimulatory effect than did any of its derivatives examined. This suggests that butyrate stimulates fibronectin synthesis specifically; 8Br‐cAMP also stimulated fibronectin synthesis. Additivity of stimulation by butyrate and by 8Br‐cAMP was observed at the saturated concentration of each; the present result indicated an independent mechanism of action for these two compounds.