Premium
Kinetics of long‐term (72 hr) calcium content during mitogen activation of cultured human T lymphocytes
Author(s) -
Wolff C. H. J.,
Åkerman K. E. O.,
Andersson L. C.
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041230108
Subject(s) - concanavalin a , egta , stimulation , in vitro , calcium , cell culture , mitogen activated protein kinase , intracellular , biology , ionophore , tissue culture , medicine , thymidine , microbiology and biotechnology , endocrinology , chemistry , biochemistry , genetics
In vitro stimulation of human blood lymphocytes with mitogen resulted in an increased intracellular content of Ca 2+ per unit cell volume. This increase in Ca 2+ content of lectin‐activated cells reached a maximum after 24 hr of culture and thereafter slowly declined. Brief treatment of cells at 24 hr of culture with the Ca 2+ ionophore A23187 in combination with EGTA resulted in a larger release of Ca 2+ from cells in mitogen‐stimulated cultures than from cells in control cultures. This indicates that the Ca 2+ is accumulated intracellularly but is readily exchangeable. At 24 hr of culture the increase in cellular Ca 2+ correlated well with the proliferative response as measured by 3 H‐thymidine incorporation. Ca 2+ influx at 24 and 48 hr of culture was markedly enhanced in the mitogenically stimulated cells as compared either to cells cultured for 1 and 72 hr or cells cultured without mitogen.