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Activation of collagen synthesis in primary culture of rat liver parenchymal cells (hepatocytes)
Author(s) -
Hata RyuIchiro,
Ninomiya Yoshifumi,
Sano Junjiro,
Konomi Hiroshi,
Hori Hisae,
Sunada Hironobu,
Tanaka Shizuko,
Kabuki Keiichiro,
Nagai Yutaka,
Tsukada Yutaka
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041220302
Subject(s) - hydroxyproline , primary culture , hepatocyte , parenchyma , incubation , type i collagen , proline , microbiology and biotechnology , albumin , chemistry , cell culture , biochemistry , in vitro , biology , endocrinology , amino acid , botany , genetics
An increase in collagen synthesis by hepatic parenchymal cells (hepatocytes) was observed during 8 days in primary culture by the quantification of total [ 3 H]hydroxyproline as a marker of total collagen synthesis and the ratio of [ 3 H]hydroxyproline in the high‐molecular‐weight fraction to total [ 3 H]hydroxyproline as a marker of collagen degradation after incubation of the cells with [ 3 H]proline for 24 h. Type analysis of the collagen produced by the cells after 8 days in culture showed the presence of type I and type III collagens in addition to the components corresponding to type IV and type V (αA and βB) collagens. Only the latter two types were found in the collagens produced by the cells after 2 days in primary culture. (a) The purity of the hepatocytes inoculated was 97%, and the majority of the contaminating small cells were erythrocytes. (b) The rate of serum albumin synthesis, which is a typical function of the hepatocytes, was constant or increased during the culture period. (c) Immuno‐electron microscopic observation indicated the production of type I collagen by the hepatocytes after 8 days in primary culture. These results are explained only by the activation of collagen synthesis in the day‐8 hepatocytes in primary culture.