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Characterization of chemically and virally transformed variants of Madin‐Darby canine kidney (MDCK) epithelial cells
Author(s) -
U H. S.,
Boerner P.,
Rindler M. J.,
Chuman L.,
Saier M. H.
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041220220
Subject(s) - in vitro , cell culture , biology , contact inhibition , ornithine decarboxylase , microbiology and biotechnology , kidney , clone (java method) , chemistry , enzyme , biochemistry , endocrinology , gene , genetics
Oncogenic derivatives of Madin‐Darby canine kidney (MDCK) cells were isolated in the nude mouse, and nononcogenic anchorage‐independent transformants were isolated in vitro following chemical mutagenesis in vitro. These transformed cell lines as well as a Moloney sarcoma virus (MSV) transformed line were characterized with respect to their serum and anchorage requirements, growth rates, final saturation densities, and sensitivities to contact inhibition. None of these in vitro growth characteristics were found to correlate with tumorigenicity in nude mice. One tumongenic clone, MDCK‐T 1 , was characterized with respect to serum‐free growth requirements, cAMP production, and ornithine decarboxylase (ODC) activity. These cells exhibited a significant reduction in the PGE 1 requirement for growth, they produced higher levels of cAMP, and they expressed a reduced level of ODC activity relative to the parental MDCK cells. These findings may reflect changes in growth control mechanisms which accompany kidney epithelial cell tumori‐genesis and suggest that the study of transformed lines derived in this manner could lead to the identification of in vitro properties which are associated with malignancy.