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Intracellular calcium redistribution and its relationship to fMet‐Leu‐Phe, leukotriene B 4 , and phorbol ester induced rabbit neutrophil degranulation
Author(s) -
Naccache P. H.,
Molski T. F. P.,
Borgeat P.,
Sha'afi R. I.
Publication year - 1985
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041220217
Subject(s) - leukotriene b4 , degranulation , calcium , chemotaxis , cytochalasin b , intracellular , chemistry , ionophore , calcium in biology , granulocyte , phorbol , cytoplasm , biophysics , biochemistry , biology , protein kinase c , immunology , enzyme , in vitro , inflammation , receptor , organic chemistry
The addition of low concentrations (<10 −7 M) of the calcium ionophore A23187 to rabbit neutrophils re'eases the intracellular pool of calcium previously shown in radioactive steady‐state and chlortetracycline fluorescence studies to be mobilized by chemotactic factors. A23187 at these concentrations elicits no functional responses from these cells. However, A23187, added before chemotactic factors such as fMet‐Leu‐Phe and leukotriene B 4 , inhibits the ability of the latter stimuli to induce, in the presence of cytochalasin B, an exocytotic release of the neutrophil's cytoplasmic granules. These results imply that the chemotactic‐factor‐induced release of intracellular calcium is a necessary event for the optimal activation of the neutrophils. Phorbol esterinduced neutrophil degranulation on the other hand is unaffected by exposure to A23187, thereby completely dissociating its mechanism of action from rises in cytoplasmic free calcium.